%0 journal article
%@ 1582-1838
%A Wang, W., Li, W., Ong, L.-L., Furlani, D., Kaminski, A., Liebold, A., Luetzow, K., Lendlein, A., Wang, J., Li, R.-K., Steinhoff, G., Ma, N.

%D 2010
%J Journal of Cellular and Molecular Medicine
%N 1-2
%P 392-402 
%R doi:10.1111/j.1582-4934.2008.00624.x
%T Localized SDF-1alpha gene release mediated by collagen substrate induces CD117+ stem cell homing
%U https://doi.org/10.1111/j.1582-4934.2008.00624.x
1-2 
%X Results: The optimized parameters for GAC were DNA dosage 10μg/cm2, molar ratio of PEI nitrogen in primary amine to DNA phosphate (N/P ratio) 4 and mass ratio of collagen to DNA (C/D ratio) 1.0. It kept cell viability above 75% and transfection efficiency around 5.8 × 105 RLU/mg protein. GAC allowed the sustained gene release up to 60 days. GAC mediated SDF-1α gene release induced migration and homing of CD117+ stem cells in vitro and in vivo significantly, and the inflammation of GAC reduced significantly two weeks after transplantation. GAC is a promising stem cell based therapeutic strategy for regenerative medicine.