%0 journal article
%@ 2159-2535
%A Burmester, A., Luthringer, B., Willumeit, R., Feyerabend, F.

%D 2014
%J Biomatter
%N 1
%P e967616 
%R doi:10.4161/21592527.2014.967616
%T Comparison of the reaction of bone-derived cells to enhanced MgCl2-salt concentrations
%U https://doi.org/10.4161/21592527.2014.967616
1 
%X Because cell lines are preferred targets in studies of non-degradable implant materials, we performed a comparative study of three osteosarcoma-derived cell lines (MG63, SaoS2 and U2OS) with primary human osteoblasts. The correlation among cell count, viability, cell size and several MgCl2 concentrations was used to examine the influence of magnesium on proliferation in vitro. Moreover, bone metabolism alterations during proliferation were investigated by analysing the expression of genes involved in osteogenesis. It was observed that for all cell types, the cell count decreases at concentrations above 10 mM MgCl2. However, detailed analysis showed that MgCl2 has a relevant but very diverse influence on proliferation and bone metabolism, depending on the cell type. Only for primary cells was a clear stimulating effect observed. Therefore, reliable results demonstrating the osteoconductivity of magnesium implants can only be achieved with primary osteoblasts.