%0 journal article
%@ 1742-7061
%A Sarem, M., Vonwil, D., Luedeke, S., Shastri, V.P.

%D 2017
%J Acta Biomaterialia

%P 285-292 
%R doi:10.1016/j.actbio.2017.05.021
%T Direct quantification of dual protein adsorption dynamics in three dimensional systems in presence of cells
%U https://doi.org/10.1016/j.actbio.2017.05.021
 
%X The biomaterial surface upon exposure to biological fluids is covered by a layer of proteins, which is modified over a period of time and dictates the fate of the biomaterial. In this study, we present and validate a new methodology for quantification of protein adsorption on to a three-dimensional polymer scaffold from unitary and binary systems, using fluorescence molecular tomography, an optical trans-illumination technique with picomolar sensitivity. In additional to being able to follow behavior of two proteins simultaneously, this methodology is also suitable for studying protein uptake in cells situated in a polymer environment. The ability to follow protein adsorption/uptake in a continuous manner opens up new possibilities to study the role of serum proteins in biomaterial compatibility.