Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococus fascians
AbstractIn a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic
bacterium, Rhodococcus fascians, were able to induce cytokine release (TNFα) from mouse peritoneal macrophages. Monoclonal
antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high
molecular (MWN100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA
variants for its determination were developed.